CURRICULUM VITAE

CURRICULUM VITAE

1.- PERSONAL INFORMATION

Name: Ferreira de Mattos, Gonzalo Rafael.

Date of Birth: XXXXXXX

Home address: XXXXXXXX. Montevideo. Uruguay.

Work address: Gral. Flores 2125. Depto. Biofisica. Facultad de Medicina. CP11800. Montevideo.

Home Phone Number: XXXXXXXXX

Work Phone Number: (5982)-9243414 ext 3203.

Fax Number: (5982)-924-8784.

E-mail: ferreiragon@gmail.com, ferreira@fmed.edu.uy

2.- ACADEMIC DEGREES

2.1.- M.D. - Faculty of Medicine, University of the Republic, Montevideo, Uruguay. 1994

2.2.- Ph.D. (Biophysics) - PEDECIBA (Program for the Development of Basic Sciences), University of the Republic, United Nations, Montevideo, Uruguay. 2003.

2.3.- Master (Biophysics) - PEDECIBA (Program for the Development of Basic Sciences), University of the Republic, United Nations, Montevideo, Uruguay. 1992.

3.- ACADEMIC SERVICE/EMPLOYMENT

3.1. CURRENT EMPLOYMENT

- Full Time Assistant Professor . Ion Channels Laboratory (Director, PI). Biophysics Dept. Faculty of Medicine. University of the Republic. Montevideo. Uruguay. (1997- ). Current Status - Under Promotion to Associate Professor.

3.2. PAST EMPLOYMENT

a.- Visiting Professor. Anatomy and Neurobiology. Salkoff´s Lab. School of Medicine. Washington University in Saint Louis. MO. USA. 63110. (2003-2005, 2006)

b.- Instructor. Dept. Of Physiology and Molecular Biophysics. Rush University. Chicago. IL. USA. (1998-99).

c.- Research Assistant, Postdoctoral Fellow. Dept. Of Physiology and Molecular Biophysics. Rush University. Chicago. IL. USA. (1995-97).

d.- Instructor . Biophysics Dept. Faculty of Medicine. University of the Republic. Montevideo. Uruguay. (1990-97).

e.- Research Fellow. Biophysics Dept. Faculty of Medicine. University of the Republic. Montevideo. Uruguay. (1987-90).

4.- HONORS AND AWARDS

4.1.- Grants funded

4.1.1. CURRENTLY FUNDED

-Effect of intoxication by metals in L-type Calcium channels and cardiac function. PDT (IBD. Interamerican Bank for Development). Uruguay. (2006-2008).

-Creation of derivatives of drugs currently used for treatment of infectious diseases that minimize side effects in cardiac muscle. Supported, Proposed and Co-guided with Pharmaceutical Companies.

4.1.2.FUNDED IN THE PAST.

i.- Research

-Relationship of voltage and calcium-dependent inactivation of L-type Calcium channels in cardiac cells and expression systems. FCE. (IBD basic sciences support) Uruguay. (2002-03).

-Functional Coupling between L-type Ca channels and BK channels. Third World Academy of Sciences. International. (2000).

ii.- Teaching

-Application of digital format techniques for teaching Biophysics. (Web design www.bioltis.fmed.edu.uy, net for students, animations used in the classroom). CSE. Uruguay. 2000.

iii.- Organization of Scientific Events

-International course “Molecular Biology of Ion channels”. PEDECIBA. Uruguay. 2002.

-International Symposium “New Perspectives in Intracellular Calcium and Calcium Sensitive channels”. Program for Technological Development and CSIC. Uruguay. 2002.

4.2.- Fellowships

- Special fellowship for Master in Biological Sciences (Biophysics). (1989). (PEDECIBA).

- International course “Transduction in Biological Systems”. (1988). CECS. Chile.

- International course “Structural basis of muscle contraction”. (1988). IVIC. Venezuela.

- Columbia Space Shuttle Program. (1981). (NASA, Organización de Estados Americanos).

4.3.- Honorary Award Services as Adjunct Honorary Professor.

- PEDECIBA. Program for The Development of Basic Sciences. United Nations, Uruguay joint Program. Graduate Education and Research Training in Sciences. (2005- ).

- CECS. Center for Scientific Studies. Physiology and Molecular Biophysics. Valdivia. Chile. (2001- ). Promoted by Dr. R. Latorre. Member of the National Academy of Sciences. USA.

5.- RESEARCH ACTIVITIES

5.1.- Publications

5.1.1. Peer Reviewed

- Insights about inactivation in L-type Calcium channels by comparing surfaces of inactivation and gating charge availability of ionic and gating currents. (2007). Budelli, G; Brum, G; Pizarro, G and Ferreira, G. *. Under submission. * corresponding author.

-High conductance Potassium channels of the Slo family. (2006). Salkoff, L; Butler, A, Ferreira, G, Santi, C, Wei, A. Nature Neuroscience (Rev): : 7(12):921-31.

-Opposite regulation of Slick and Slack K+ channels by Neuromodulators. (2006). Santi, C*, Ferreira, G*, Yang, B*, Valeswara-Rao, G, Butler, A, Wei, A, Kaczmarek, L, and Salkoff, L Accepted J. Neuroscience. *, shared first authorship. May 10 issue. 26 (19):5059-68

-Potassium Channels in C. elegans. L. Salkoff, B. Baban, A. Butler, G. Fawcett, G. Ferreira, C.M. Santi, & A. Wei . (2005). Wormbook, 30 Dec.

-Two components of voltage-dependent inactivation in Ca_v 1.2 channels revealed by its gating currents. *G.Ferreira, E.Ríos y N.Reyes. (2003). Biophys J. 84(6):3662-78. * corresponding author.

-Effects of the enantiomers of BayK 8644 on the charge movement of L-type Ca channels in guinea-pig ventricular myocytes. (2003). Artigas P, Ferreira G, Reyes N, Brum G, Pizarro G. J Membr Biol. 193(3):215-27

-New Disguises for an Old Channel: Voltage and Calcium-Activated K⁺ (MaxiK) Channels beta subunits. Patricio Orio, Patricio Rojas, Gonzalo Ferreira and Ramón Latorre. News in Physiological Sciences. (2002). 17:156-161.

-Inactivation of gating currents of L-type calcium channels. Specific role of the a₂d subunit. Shirokov, R; Ferreira, G; Yi, J and Rios, E. (1998). J. Gen. Physiol. 111: 807-823.

-Ion-dependent inactivation of barium current through L-type calcium channels. Ferreira, G; Yi, J; Rios, E; and Shirokov, R. (1997). J Gen Physiol. 109: 449‑461.

-Butanedione Monoxime promotes voltage-dependent inactivation of L-type calcium channels in heart. Effects on gating currents. Ferreira, G; Artigas, P; Pizarro, G and Brum, G. (1997). J Mol Cell Cardiol. 29(2): 777‑787.

-Comparison of the effects of BDM on L-type Ca channels of cardiac and skeletal muscle. Gonzalo Ferreira, Pablo Artigas, Rafael De Armas, Gonzalo Pizarro and Gustavo Brum In Calcium and cellular metabolism: Transport and Regulation. Ed. J.R.Sotelo & J.C.Benech. Published by Plenum Pub Corp. Publication date: June 1997. ISBN: 0306455943.

-Inactivacion voltaje-dependiente en canales de Ca2+ L (Cav1.2) expresados en celulas tsA 201. (Translation – Voltage-dependent inactivation in L-type Ca2+ channels (Cav1.2), expressed in tsA 201 cells). Ph.D. Thesis. (PEDECIBA Library). Faculty of Medicine, Montevideo, Uruguay.

-Registro y caracterización del movimiento de carga intramembrana en miocitos aislados de miocardio ventricular de cobayo. (Translation - Record and characterization of the intramembrane charge movement in myocytes isolated from the guinea-pig myocardial ventricle). M.Sc. Thesis. (PEDECIBA Library). Faculty of Medicine, Montevideo, Uruguay.

5.1.2. Abstracts (selected year 1999 to date only).

-Heteromerization of mammalian and nematode Slo2 channels expressed in Xenopus Oocytes. (2006). Ferreira, G; Budelli, G; Butler, A; Wei, A; Santi, C and Salkoff, L. SUB Molecular Biology V Meeting.

-Two novel mammalian high conductance potassium channels are oppositely modulated by PKC. Ferreira, G; Santi, C; Butler, A; Wei, A and Salkoff, L. Biophys. J. Meeting scheduled for 2005.

-The structural requirements for inhibition by PKC in Slo2.1 channels are located in the channels’s tail. Santi, C; Ferreira, G; Butler, A; Wei, A and Salkoff, L. Biophys. J. Meeting scheduled for 2005.

-Activation of BK channels by local Ca2+ entry through L-type Ca2+ channels, coexpressed in tsA 201 cells. N.Reyes, L.Salkoff, A.Butler, G.Pizarro, G.Brum and G.Ferreira. XIV International Biophysics Congress (IUPAB), 2002, 103p.

-Efecto de cationes extracelulares en el componente rápido de inactivaci{on dependiente de voltaje en Cav1.2 expresado en células tsA 201. G. Ferreira, N.Reyes, G.Budelli, R. De Armas, G.Brum y E.Ríos. X Congreso Sociedad Uruguaya de Biociencias (SUB), 2002, B41.

-Effects of partial deletions of S3 segment in Shaker K+ channel activation kinetics. C.Gonzalez, G.Ferreira, E.Rosenmann, F. Bezanilla, O.Alvarez and R.Latorre. Biophys. J. 2002. 82:A234.

-Modulation by Ca2+ and Mg2+ of mammalian muscle Ca2+ sparks. J.Zhou, A.Gonzalez, G. Ferreira, R.Segura, E.Rios, J.Yi and G.Brum. Biophys.J. 2002. 82:A510.

-Functional coupling between L-type Calcium channels and BK channels coexpressed in tsA 201 cells. N. Reyes, G. Pizarro, G. Brum, L.Salkoff and G.Ferreira. Biophys.J. 2001. 80:A209.

-Correlation of surfaces of inactivation of ionic and gating currents in L-type Ca channels expressed in tsA 201 cell.s. G.Ferreira, N.Reyes, G.Pizarro, G.Brum and E.Ríos. Biophys.J. 2001. 80:A620.

-Ausencia de inactivaciòn preferencial de estados cerrados en canales de Ca2+ L. Ferreira, G; Reyes, N; Pizarro, G; Brum, G and Rìos, E. SUB 2000. Libro de resùmenes.

-Coexpresiòn de canales de Ca2+ L y K+ activados por Ca2+ (BK) en cèlulas tsA 201. Ferreira, G; Reyes, N; Pizarro, G; Brum, G; Salkoff, L and Brandl, F. SUB 2000. Libro de resùmenes.

-Efecto de S(-) BayK8644 sobre las corrientes de compuerta (Ig) del canal de Ca2+ L cardìaco. Artigas, P; Ferreira, G; Reyes, N; Brum, G and Pizarro, G. SUB 2000. Libro de resùmenes.

-Corrientes de membrana y transitorios de Ca2+ en cèlulas musculares esquelèticas aisladas de la cola del renacuajo. Ferreira, G; Reyes, N; Rìos, E; Pizarro, G and Brum, G. SUB 2000. Libro de resùmenes.

-Fast and slow components of voltage dependent inactivation in L-type Ca2+ channels. Ferreira, G; Yi, J and Ríos, E. Biophys. J. (1999). 76: A340.

5.2.- Scientific Activities and Events

5.2.1.- Invited Lectures (selected year 1999 to date)

-Side effects of drugs in cardiac calcium channels and new drug design minimizing those problems. Invited by Pharmaceutical Companies. (2006). Gautier Laboratories.

-Secondary Channelopathies, Isaac’s Syndrome (2006)– Institute of Neurology. Hospital. Montevideo. Uruguay. Invited by Institute of Neurology.

-Modulation of Slo2 channels expressed in Xenopus oocytes (2005)- SUB regional meeting. Uruguay. Invited by SUB.

- Functional coupling between L-type Ca2+ channels and BK channels expressed in tsA 201 cells. (2001).

Centro de Estudios Cientificos. Chile.

-Inactivation in L-type Ca channels. April 2001.FASEB meeting (Experimental Biology). Orlando. Florida. USA. Invited by American Physiological Society.

-Voltage dependent inactivation in L-type Ca2+ channels. (2001). Rush University. Chicago. USA.

-Voltage dependent inactivation mechanisms in L-type Ca channels. International Symposia “Ion channels, a glimpse to the future”. (2000). Invited by CECS, Valdivia, Chile.

5.2.2.- Meetings Assistance

- 40^th , 41^th , 42^th, 43^th, 45^th , 47^th , 48^th Annual Meeting Biophysical Society. USA. (1996, 97, 98, 99, 01, 04, 05).

- Experimental Biology Meeting. (2001). Orlando. Florida. USA.

- International Union of Pure and Applied Biophysics Meeting (2002).

- Simposio Satélite de el XXXVII Congreso Mexicano de Ciencias Fisiológicas. (1994). Cancún, México.

- XVIII Congreso Latinoamericano de Ciencias Fisiológicas. (1994). Montevideo, Uruguay.

- Society of Uruguayan Bioscientists (SUB). Uruguay. 2000, 2001, 2005, 2006.

5.2.3. – Meetings Organized.

- FIELD OF WORK - Calcium and Potassium channels.

- GENERAL EXPERIMENTAL APPROACHES AND TECHNIQUES USED: Electrophysiology, Molecular Biology, Cardiac Physiology, Neurobiology, Skeletal Muscle, Intracellular Calcium, Genetics, Bioinformatics, Protein Dynamics (FRET), Biochemistry, Pharmacology and Signal Transduction.

- LAB. DIRECTOR (PI). Ion Channels Laboratory. Biophysics Dept. School of Medicine. Uruguay. (2002 - ).

6.- TEACHING ACTIVITIES

6.1.- Courses (Faculty of Medicine, Montevideo, Uruguay)

6.1.1. Undegraduates

- Biophysics. Lectures (91- 2006) Ion Channels, Muscle Physiology. Workshops (86-91) for students of the career of Dr. in Medicine.

- Quantitative Methods in Biology (1987).

- Cell Biology (Membranes and Radiobiology). Lectures (91-95) and Workshops (86-91) for students of the career of Dr. in Medicine and nurses school.

-Physiology (Excitable Tissues, Cardiophysiology, Pulmonary physiology). Lectures (Excitable Tissues) (91 to 95 - 99 to 2002) and Workshops (86-91) for students of the career of Dr. in Medicine.

-Electrocardiography (2002). Lectures for students of the career of Dr. in Medicine.

6.1.2. Graduate

6.1.2.1 National

- Advanced Biophysics (Biophysics II, H and H model discussion, programming, Single Channel, stochastic processes, Gating and Permeation (critical papers and current problems). PEDECIBA. MSc, PhD Programs. Biophysics. 2005-2006. (One month)

- Cell Physiology (Ion Channels Module, Molecular Biology, 3D structure, Pharm, current problems, future perspectives, channels in non-excitable cells) PEDECIBA. 2005. Two weeks.

6.1.2.2 International

- Molecular Biology of Ion channels. 2002. PEDECIBA, IUPAB Satellite.

- Latin-American School of Neurosciences. (2000-2003). Lectures, Seminar. Workshop. Molecular basis of excitability and gating of calcium channels. IIBCE. Montevideo. Uruguay.

- UNESCO.IBRO.ICRO. “Novel Spectroscopic Approaches to Neurobiology”. (2000). Faculty Teaching Staff. Lectures. Seminar. Workshop. Ion channel gating and Calcium channel inactivation. INIBIBB. IBRO. ICRO. Bahia Blanca. Argentina.

6.2.- Laboratory training of students (Formation of Human Resources).

-1994-95: Patch clamp and isolation of myocytes. Pablo Artigas. Currently at Rockefeller University. NY. USA.

-1999-02: Patch Clamp, Tissue culture, Basic Molecular Biology. Nicolas Reyes. Currently at Cornell University. NY. USA, from Rockefeller University. Thesis. Functional coupling between L-type Calcium channels and BK channels coexpressed in tsA 201 cells. Supervisors: G.Ferreira and G.Pizarro developing a project submitted by G. Ferreira.

-2002: Patch clamp setups, numerical methods applied to ion channels. Gonzalo Budelli. Currently in Washington University, StL, MO, USA.

-2003-2005: Isolation and injection of oocytes in Xenopus laevis. Johnatan Garst-Orozco. Currently accepted in several top universities and programmes in the US.

-2003-2005: Isolation and injection of oocytes in Xenopus laevis. Marisa Jackson. Currently accepted as a MD, PhD student, Northwestern University, Chicago, IL, USA.

-2003-2005: Isolation and injection of oocytes in Xenopus laevis. Jennifer Luke. Currently accepted as a MD student. Washington University. Anatomy and Neurobiology.

-2006: Isolation of cardiac myocytes, patch clamp, basic general biophysics and electrophysiology: Juan Rompani, Alejandro Schmidt, Diego Gonzalez, Magdalena Silvera, Valeria Ochoa. Under training and participating continously in all currently funded projects.

6.3.- Letters of recommendation solicited by students and colleagues that I have worked/taught/interacted with

-Nicolas Reyes – 2001 and 2006 – Rockefeller University admittance. Currently at Cornell University, NY, USA. Member of the Society of General Physiology council.

-Karen Lawrence – 2005 – Currently at the Program in Neuroscience. WUSTl. MO. USA.

-Carlos Gonzalez – 2003 y 2005 – PhD, Chile. Currently a Postdoc at NIH, Maryland, USA.

-Patricio Rojas – 2005 -Currently a Postdoc at Washington University.

-Marisa Jackson – 2004 – Currently in the MD PhD Program at Northwestern University, Chicago, IL, USA.

-Fabian Lopez. – 2003 – Currently in the PhD Program at University of Chile.

-Gonzalo Budelli. – 2006 - Currently at the Program in Neuroscience. WUSTl. MO. USA.

-Johnathan Garst-Orozco – 2006 – Currently accepted at the Programs in Neuroscience at Harvard University and Washington University.

6.4.- Tribunal

- 2001-2002 and 2005. Biophysics Department Advisor and Admittance Committee. Faculty of Medicine. Montevideo. Uruguay.

-2006- Graduate Students Thesis Referee. Diabetic cardiomyopathy – Sandra Mai. Br in Biochemistry. School of Sciences. Uruguay.

6.5.- Short Textbooks and Syllabus

-Ionic Channels. (1989). G.Brum and G.Ferreira. Oficina del libro AEM.

-Excitation-Contraction Coupling in Skeletal Muscle. (1989). G.Brum and G.Ferreira. Oficina del libro AEM.

-Syllabus. (2005) and (2006). Complemented with Web resources developed by G.Ferreira.

7.- EDUCATIONAL BACKGROUND (extracurricular courses, taken in addition to those necessary to obtain degrees mentioned above)

7.1.- Uruguay

-English. (1974-81). Institute Anglo-Uruguayo.

-Biomathematics. (1983). School of Science.

-Algebra. (1985). School of Science.

-Mathematical Analysis. (1985). School of Science.

-Mathematical Models. (1987). School of Medicine.

-Informatics applied to biology. (1987). School of Medicine.

-Statistics in Medicine. (1987). School of Medicine.

-Ionic Channels in Biological Membranes. (1988). School of Medicine.

-Electronics in Biology. (1989). School of Engineering.

-Mathematics for biophysicists. (1989). School of Medicine and School of Sciences.

-Axonal Transport. (1989). Institute Clemente Estable.

-Molecular and Cell Biology. (1990-1991). School of Sciences.

-Molecular basis of excitability. (1991). PEDECIBA.

-Population Dynamics. (1992). PEDECIBA.

-Transgenic rodents. (2006). Institute Pasteur.

-Structural Bioinformatics and Molecular Dynamics. (2006). School of Chemistry.

-Biophysical theory behind structural bioinformatics. (2007). Institute Pasteur.

7.2.- International

-Structural Basis of muscular contraction. (1988). IVIC Venezuela.

-Transduction in Biological Systems. (1988). CECS. Chile.

-Molecular and cell biology of ion channels. (1994). Cancún. Mexico.

-Molecular approaches to ion channel biology.(1997). Cold Spring Harbor. New York. USA.

-Bioinformatic approaches to Human Genome Resources. (2006). Wellcome Trust, Sanger Institute, Cambridge University (UK)..

8.- PROFESSIONAL AFFILIATIONS